Biological and histopathological investigations of moclobemide on injured ovarian tissue following induction of ischemia-reperfusion in rats

The effects of moclobemide on damaged ovarian tissue induced by is-chemia-reperfusion and damaged contralateral ovarian tissue were investigated in rats, biochemically and histologically. In this experimental study, 40 rats were equally divided into four groups: 10 mg/kg moclobemide, 20 mg/kg moclobemide, ischemia/reperfusion control, and intact control groups. A 2-2.5-cm-long vertical incision was made in the lower abdomen of each rat in order to reach the ovaries, after which a vascular clip was placed on the lower side of the right ovary of each animal in the two treatment groups and the ischemia-reperfusion control group, but not in the healthy [intact control] animal group. The purpose of this procedure was to create ischemia over the course of three hours, then the clips were unclamped to provide reperfusion for the next two hours. At the end of the two hours of reperfusion, all the animals were killed by high-dose anaesthesia and their ovaries were taken and subjected to histological and biochemical [malondialdehyde, nitric oxide, glutathione] studies. The obtained results showed that moclobemide suppressed nitric oxide and malondialdehyde production in the ischemia - reperfusion damage area, and prevented the decrease in endogenous antioxidant levels [glutathione] in the rat ovarian tissue. Moclobemide also prevented infiltration of leukocytes to the ovarian tissue. These results showed that moclobemide protected ovarian tissue against ischemia-reperfusion injury. This study shows that moclobemide represses malondialdehyde and nitric oxide production in the rat ovarian tissue subjected to ischemia-reperfusion injury and keeps the endogenous antioxidant glutathione level from decreasing. Moclobemide also inhibits leukocytic migration into ovarian tissue following ischemia-reperfusion injury. From these results, it is suggested that moclobemide can be used in the treatment of ovarian ischemia-reperfusion injury

effect of metyrosine/prednisolone combination to oophorectomy-induced osteoporosis

Osteoporosis is a chronic disease characterized by a decrease in bone mineral density [BMD] and corruption of the microarchitectural structure of bone tissue. It was investigated whether methylprednisolone had a favorable effect on osteoporotic bone tissue in Oophorectomy induced osteoporotic rats whose endogenous adrenaline levels are suppressed with metyrosine. Bone Mineral Density, number of osteoblast-osteoclast, bone osteocalcin levels and alkaline phosphatase [ALP] measurements were performed. Obtained results were compared with that of alendronate. Oophorectomy induced osteoporosis was exacerbated by methylprednisolone. Alentronate prevented ovariectomised induced osteoporosis, but it couldn't prevent methylprednisolone +ovariectomised induced osteoporosis in rats. Combined treatment with methylprednisolon and metyrosine was the best treatment for preventing osteoporosis but metyrosine alone couldn't prevent osteoporosis in ovariectomised rats

Experimental comparison of bovine-derived xenograft, xenograft-autologous bone marrow and autogenous bone graft for the treatment of bony defects in the rabbit ulna

The purpose of this study was to determine whether bone marrow grafted percutanously has an effect on the healing of bony defects filled with bone-derived xenografts. Materials and Eighty New Zealand Al bino rabbits with an average age of approximately 1 year were divided into 4 groups, each consisting of 20 animals. Bony defect was induced in the ulnas of all rabbits by excising a 1-cm-long bone segment from the 3-cm proximal segment of the right distal radioulnar joint. Bone defects were treated simultaneously with bovine-derived xenograft, a combination of xenograft and bone marrow or on the 5th day following the filling of the segment with the xenograft and autogenous bone graft. Treatment results obtained for each application type were compared with each other with regard to the radiological, biochemical and histological criteria. No significant statistical differences were determined between the groups in their 15th-day radiographs. The group treated with only xenograft from the first month onwards presented with the worst results and was significantly different from the other groups with respect to all evaluation criteria. No statistically significant difference was determined between rabbits treated with xenograft and bone marrow combination and with autogenous bone graft. This study revealed that when xenografts are combined with autologous bone marrow, their incorpora-tion into the host bed accelerates significantly

Histopathologic evaluation of neurogenic pulmonary edema after subarachnoid hemorrhage in rabbits

To investigate the effects of subarachnoid hemorrhage [SAH] on lung tissue. We conducted this study on 20 rabbits in the Ataturk University Medical Faculty, Erzurum, Turkey in 2005. Experimental SAH was applied to all animals under general anesthesia. After 20 days, all animals were sacrificed. Their lungs were examined histopathologically. Foamy hemorrhagic parenchymal lesions, alveolar rupture, and subintimal fluid collection in the pulmonary vasculature were observed in the lungs of the non-surviving animals. However, minimal changes were found in the lungs of the surviving animals [p<0.01]. Our results suggest that luminal narrowing of the lung vessels due to subintimal fluid collection plays an important role in the development of pulmonary hypertension and neurogenic pulmonary edema in SAH